Your proposal should be organized as follows:
Abstract: The abstract should summarize your proposal. Include one sentence to introduce the problem you are investigating, why this problem is significant, the hypothesis to be tested, a brief summary of experiments that you wish to conduct and a single concluding sentence.
Introduction: The introduction discusses the background and significance of the problem you are investigating. Lead the reader from the general to the specific. For example, if you want to write about the role that Brca1 mutations play in breast cancer pathogenesis, talk first about the significance of breast cancer as a disease in the US/world population, then about familial breast cancer as a small subset of breast cancers in general, then about discovery of Brca1 mutations in familial breast cancer, then Brca1’s normal functions in DNA repair, then about how Brca1 mutations result in damaged DNA and onset of familial breast cancer, etc. Definitely include figures with properly labeled text boxes (designated as Figure 1, Figure 2, etc) here to better illustrate your points and help your reader wade through unfamiliar science.
Hypothesis: Formulate a hypothesis that will be tested in your grant proposal. Remember, you are doing hypothesis-driven research so there should be a hypothesis to be tested! The hypothesis should be focused, concise and flow logically from the introduction. For example, your hypothesis could be “I hypothesize that overexpressing wild type Brca1 in Brca1 null tumor cells will prevent metastatic spread in a mouse xenograph model.” Based on your hypothesis, your Specific Aims section should be geared to support it. The hypothesis is stated in one sentence in the proposal.
Specific Aims (listed as Specific Aim 1, Specific Aim 2): This is where you will want to work with your mentor to craft the experimental portion of your proposal. Propose two original specific aims to test your hypothesis. Don’t propose more than two aims-you will NOT have enough time to do more. In the example presented, Specific Aim 1 might be “To determine the oncogenic potential of Brca1 null cell lines expressing wild type Brca1 cDNA”. Specific aim 2 might be “To determine the metastatic potential of Brca1 null cells that express WT Brca1”. You do not have to go into extensive technical details, just enough for the reader to understand what you propose to do. The best aims yield mechanistic insights-that is, experiments proposed address some mechanisms of biology. A less desirable aim proposes correlative experiments that does not address mechanistically how BRCA1 mutations generate cancer. It is also very important that the two aims are related but NOT interdependent. What this means is that if Aim 1 doesn’t work, Aim 2 is not automatically dead. For example, say you propose in Aim 1 to generate a BRCA1 knockout mouse model, and in Aim 2 you will take tissues from this mouse to do experiments. If knocking out BRCA1 results in early embryonic death, you will never get a mouse that yields tissues for Aim 2. You can include some of your mentor’s data here as “Preliminary data”. Remember to carefully cite all your sources.
Potential pitfalls and alternative strategies: This is a very important part of any proposal. This is where you want to discuss the experiments you propose in Aims 1 and 2. Remember, no experiment is perfect. Are there any reasons why experiments you proposed might not work? Why? What will you do to resolve this? What are other possible strategies you might use if your experiments don’t work? If a reviewer spots these deficiencies and you don’t propose methods to correct them, your proposal will not get funded. You will want to work with your mentor to write this section.
References: Cite all references, including unpublished data from your mentor. Last, First, (year), Title, Journal, volume, pages.